access array microfluidic chip (fluidigm)
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fluidigm
access array microfluidic chip
Access Array Microfluidic Chip, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/access+array+microfluidic+chips/Access+Array/pm40428411-84-8-7
Average 93 stars, based on 843 article reviews
Access Array Microfluidic Chip, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/access+array+microfluidic+chips/Access+Array/pm40428411-84-8-7
Average 93 stars, based on 843 article reviews
access array microfluidic chip - by Bioz Stars,
2026-10
93/100 stars
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Purification:Article Title: Comparison of NK alloreactivity prediction models based on KIR-MHC interactions in haematopoeitic stem cell transplantation Article Snippet: .. After purification with SPRI - solid phase reversible immobilization - select beads AMPure XP (BeckmanCoulter, Brea, USA), concentration of the pool was checked via qPCR using an ECO Real-Time PCR cycler (Illumina, San Diego, USA) HLA genotyping: After unique indexing and addition of adapter sequences of each sample, PCR for exon 2 and exon 3 amplification was performed on Fluidigm Concentration Assay:Article Title: Comparison of NK alloreactivity prediction models based on KIR-MHC interactions in haematopoeitic stem cell transplantation Article Snippet: .. After purification with SPRI - solid phase reversible immobilization - select beads AMPure XP (BeckmanCoulter, Brea, USA), concentration of the pool was checked via qPCR using an ECO Real-Time PCR cycler (Illumina, San Diego, USA) HLA genotyping: After unique indexing and addition of adapter sequences of each sample, PCR for exon 2 and exon 3 amplification was performed on Fluidigm Real-time Polymerase Chain Reaction:Article Title: Comparison of NK alloreactivity prediction models based on KIR-MHC interactions in haematopoeitic stem cell transplantation Article Snippet: .. After purification with SPRI - solid phase reversible immobilization - select beads AMPure XP (BeckmanCoulter, Brea, USA), concentration of the pool was checked via qPCR using an ECO Real-Time PCR cycler (Illumina, San Diego, USA) HLA genotyping: After unique indexing and addition of adapter sequences of each sample, PCR for exon 2 and exon 3 amplification was performed on Fluidigm Polymerase Chain Reaction:Article Title: Comparison of NK alloreactivity prediction models based on KIR-MHC interactions in haematopoeitic stem cell transplantation Article Snippet: .. After purification with SPRI - solid phase reversible immobilization - select beads AMPure XP (BeckmanCoulter, Brea, USA), concentration of the pool was checked via qPCR using an ECO Real-Time PCR cycler (Illumina, San Diego, USA) HLA genotyping: After unique indexing and addition of adapter sequences of each sample, PCR for exon 2 and exon 3 amplification was performed on Fluidigm Article Title: MEDULLOBLASTOMA EXOME SEQUENCING UNCOVERS SUBTYPE-SPECIFIC SOMATIC MUTATIONS Article Snippet: .. For validation, PCR on Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing. Article Snippet: .. Results: We have established a straightforward three-step workflow for high-throughput HLA typing: Exons 2 and 3 of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 are amplified by PCR on Fluidigm Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing. Article Snippet: .. HLA-specific DNA amplification by PCR is performed on Fluidigm Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing Article Snippet: .. We have established a straightforward three-step workflow for high-throughput HLA typing: Exons 2 and 3 of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 are amplified by PCR on Fluidigm Article Title: Prediction of spurious HLA class II typing results using probabilistic classification. Article Snippet: While modern high-throughput sequence-based HLA genotyping methods generally provide highly accurate typing results, artefacts may nonetheless arise for numerous reasons, such as sample contamination, sequencing errors, read misalignments, or PCR amplification biases.. To help detecting spurious typing results, we tested the performance of two probabilistic classifiers (binary logistic regression and random forest models) based on population-specific genotype frequencies.. We trained the model using high-resolution typing results for HLADRB1, DQB1, and DPB1 from large samples of German, Polish and UK-based donors. Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing Article Snippet: .. HLA-specific DNA amplification by PCR is performed on Fluidigm Amplification:Article Title: Comparison of NK alloreactivity prediction models based on KIR-MHC interactions in haematopoeitic stem cell transplantation Article Snippet: .. After purification with SPRI - solid phase reversible immobilization - select beads AMPure XP (BeckmanCoulter, Brea, USA), concentration of the pool was checked via qPCR using an ECO Real-Time PCR cycler (Illumina, San Diego, USA) HLA genotyping: After unique indexing and addition of adapter sequences of each sample, PCR for exon 2 and exon 3 amplification was performed on Fluidigm Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing. Article Snippet: .. Results: We have established a straightforward three-step workflow for high-throughput HLA typing: Exons 2 and 3 of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 are amplified by PCR on Fluidigm Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing Article Snippet: .. We have established a straightforward three-step workflow for high-throughput HLA typing: Exons 2 and 3 of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 are amplified by PCR on Fluidigm Biomarker Discovery:Article Title: MEDULLOBLASTOMA EXOME SEQUENCING UNCOVERS SUBTYPE-SPECIFIC SOMATIC MUTATIONS Article Snippet: .. For validation, PCR on Sequencing:Article Title: MEDULLOBLASTOMA EXOME SEQUENCING UNCOVERS SUBTYPE-SPECIFIC SOMATIC MUTATIONS Article Snippet: .. For validation, PCR on Immunopeptidomics:Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing. Article Snippet: .. Results: We have established a straightforward three-step workflow for high-throughput HLA typing: Exons 2 and 3 of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 are amplified by PCR on Fluidigm Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing Article Snippet: .. We have established a straightforward three-step workflow for high-throughput HLA typing: Exons 2 and 3 of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 are amplified by PCR on Fluidigm DNA Amplification:Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing. Article Snippet: .. HLA-specific DNA amplification by PCR is performed on Fluidigm Article Title: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing Article Snippet: .. HLA-specific DNA amplification by PCR is performed on Fluidigm Amplicon Sequencing:Article Title: Prediction of spurious HLA class II typing results using probabilistic classification. Article Snippet: While modern high-throughput sequence-based HLA genotyping methods generally provide highly accurate typing results, artefacts may nonetheless arise for numerous reasons, such as sample contamination, sequencing errors, read misalignments, or PCR amplification biases.. To help detecting spurious typing results, we tested the performance of two probabilistic classifiers (binary logistic regression and random forest models) based on population-specific genotype frequencies.. We trained the model using high-resolution typing results for HLADRB1, DQB1, and DPB1 from large samples of German, Polish and UK-based donors. |